masson  (StatLab Medical Products Inc)

Journal: Signal Transduction and Targeted Therapy

Article Title: MAPK14 converges on key transcriptional machinery to promote vascular smooth muscle cell degeneration in abdominal aortic aneurysm

doi: 10.1038/s41392-025-02540-0

Figure Lengend Snippet: Deficiency of MAPK14 in VSMCs impedes Ang II-induced aortic remodeling and VSMC degeneration. Mapk14 WT ( Mapk14 f/f / Apoe -/- ) and Mapk14 KO ( Sm22- Cre +/- / Mapk14 f/f /Apoe -/- ) mice were infused with Angiotensin II (Ang II) for 7 days, and abdominal aortas (AAs) were isolated for the following experiments. a Representative images of hematoxylin and eosin (H&E), Masson’s trichrome, and Picrosirius Red staining of cross sections of AAs from the indicated mice. b Quantification of vessel wall thickness (medial and adventitial layers) via H&E staining ( n = 6 per group). c Quantification of fibrosis via Masson’s trichrome and Picrosirius Red staining ( n = 6 per group). d Representative immunofluorescence images of COL1 (left) and dihydroethidium (DHE) staining (right) in AAs from the indicated mice. e , f Quantification of the COL1-positive area ( e ) and DHE positive area ( f ) in AAs ( n = 6 per group). g Representative Western blot images of the indicated proteins in AAs from the indicated mice. h Quantification of the levels of the indicated proteins in the indicated groups ( n = 7 per group for HSPA5, PBH4, ATF4, and cl-CASP3; n = 4 per group for ATF6). i Representative immunofluorescence images showing PBH4, HSPA5, cl-CASP3 (green), ACTA2 (red), and DAPI (blue) in cross-sections of AAs. j Quantification of the positive area of the indicated proteins within the media layer of AAs ( n = 4 per group). k Representative transmission electron microscopy (TEM) images showing swollen mitochondria and enlarged endoplasmic reticulum (ER) in Mapk14 WT compared with Mapk14 KO mice ( n = 3 per group). Red arrows indicate swollen mitochondria, and white arrows indicate enlarged ER. The data were analyzed via Student’s t tests for all quantifications. L lumen; M medial layer

Article Snippet: Paraffin-embedded AA cross sections (5 μm) were deparaffinized, rehydrated, and then subjected to standard hematoxylin and eosin (H&E), Masson’s trichrome (StatLab, KTMTR2), or Picrosirius Red (Abcam, ab150681) staining for morphometric analysis.

Techniques: Isolation, Staining, Immunofluorescence, Western Blot, Transmission Assay, Electron Microscopy

Journal: Bioactive Materials

Article Title: A paintable and adhesive hydrogel cardiac patch with sustained release of ANGPTL4 for infarcted heart repair

doi: 10.1016/j.bioactmat.2023.08.020

Figure Lengend Snippet: Effects of gelatin/dex-ald hydrogel cardiac patches on heart functions and remodeling after MI. (A) Photographs of experimental groups (sham, MI, gelatin/dex-ald, and A4/gelatin/dex-ald). (B) Representative echocardiographic images after 2 weeks MI and treatment. (C, D) The LV function parameters (EF and FS) in various groups after 2 weeks MI. (E) Representative heart photographs and Masson's trichrome staining images of the isolated hearts after 2 weeks MI and treatment. Arrows indicate the hydrogels remaining on heart. (F) Fibrosis area and (F) LV wall thickness in each group. n = 4 (sham), n = 5 (MI), n = 5 (gelatin/dex-ald), and n = 5 (A4/gelatin/dex-ald). Statistical significance was analyzed by one-way ANOVA followed by a Tukey analysis. *p < 0.05.

Article Snippet: Cardiac fibrosis was assessed using Masson's trichrome staining (ab150686; Abcam, Cambridge, UK), and fibrotic regions were assessed using the NIS-Elements Advanced Research tool to visualize blue-stained fibrosis deposits (Nikon, Japan).

Techniques: Staining, Isolation

Journal: Bioactive Materials

Article Title: A paintable and adhesive hydrogel cardiac patch with sustained release of ANGPTL4 for infarcted heart repair

doi: 10.1016/j.bioactmat.2023.08.020

Figure Lengend Snippet: Representative immunofluorescence images of rat heart tissues 2 weeks after MI. (A) Immunofluorescence staining of vWF (an endothelial marker, green) and α-SMA (a vascular protein marker, red) in the border zone. (B) Immunofluorescence staining of CD68 (a macrophage marker, green) and CD206 (an anti-inflammatory macrophage marker, red) in the border zone. Yellow color in the fluorescence images indicate co-localization of CD68 and CD206. Upper images are the corresponding images of Masson's Trichrome staining in each group (A and B).

Article Snippet: Cardiac fibrosis was assessed using Masson's trichrome staining (ab150686; Abcam, Cambridge, UK), and fibrotic regions were assessed using the NIS-Elements Advanced Research tool to visualize blue-stained fibrosis deposits (Nikon, Japan).

Techniques: Immunofluorescence, Staining, Marker, Fluorescence